Today, I reread Fulco et al's 2019 paper on "Activity-by-contact model of enhancer–promoter regulation from thousands of CRISPR perturbation" https://www.nature.com/articles/s41588-019-0538-0 Still one of my favorites, because the authors combined innovative functional genomics with down-to-earth computational approaches - and obtained a highly usable method to predict enhancer -> gene interactions (the ABC model). #functionalgenomics #epigenetics #literature #science
NatureActivity-by-contact model of enhancer–promoter regulation from thousands of CRISPR perturbations - Nature GeneticsCombining CRISPRi-FlowFISH to perturb enhancers with an activity-by-contact model to predict complex connections allows systematic mapping of enhancer–gene connections in a given cell type, on the basis of chromatin-state measurements.
), but I finally updated Crispulator.jl to work with 