micronicle LAB<p>Experimenting with <a href="https://mastodon.social/tags/ThT" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>ThT</span></a> stain on <a href="https://mastodon.social/tags/spider" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>spider</span></a> webs to see if it successfully bonds to the beta chain <a href="https://mastodon.social/tags/fibrin" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>fibrin</span></a> / <a href="https://mastodon.social/tags/amyloid" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>amyloid</span></a> like components. I've applied the stain and naturally under 460nm webs fluoresce the expected green. I'm waiting for the sample to completely dry before differentiating with alcohol and then using a water based mount to finally disassociate the unbounded stain from the webs and other artefacts. All being so, we might be left with ThT stained <a href="https://mastodon.social/tags/spidroins" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>spidroins</span></a> <a href="https://mastodon.social/tags/science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>science</span></a> <a href="https://mastodon.social/tags/biology" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>biology</span></a> <a href="https://mastodon.social/tags/microscopy" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>microscopy</span></a></p>