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#transcriptomeanalysis

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Frank Aylward<p>If you are annotating genes in a eukaryotic genome with <a href="https://genomic.social/tags/rnaseq" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>rnaseq</span></a> , how do you handle antisense transcripts? It seems like they are quite common in many situations. </p><p>I'm using tophat + stringtie, and sometimes I see features being called on the wrong strand. </p><p><a href="https://genomic.social/tags/transcriptome" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>transcriptome</span></a> <a href="https://genomic.social/tags/transcriptomeAnalysis" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>transcriptomeAnalysis</span></a> <a href="https://genomic.social/tags/RNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>RNA</span></a> <a href="https://genomic.social/tags/mRNA" class="mention hashtag" rel="nofollow noopener" target="_blank">#<span>mRNA</span></a></p>